uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application
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چکیده
منابع مشابه
uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application
UNLABELLED uMelt(SM) is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest neighbor stacking energies, loop entropy effects, cation (monovalent and Mg(++)) concentrations and a temperature range. Using an accelerated partit...
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Melting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single-nucleotide variant (SNV) after PCR is the composite of four duplexes: two matched homoduplexes and two mismatched heteroduplexes. To better predict the shap...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2011
ISSN: 1460-2059,1367-4803
DOI: 10.1093/bioinformatics/btr065